Nonlinear Microscopy for Imaging Deep in Scattering Tissue

Tuesday, April 30, 2019 - 3:10pm - 4:00pm
Lind 305
Chris Xu (Cornell University)
Over the last two decades, nonlinear microscopy has created a renaissance in the brain imaging community. It has changed how we visualize neurons by providing high-resolution, non-invasive imaging capability deep within intact brain tissue. Nonlinear imaging will likely play an essential role in understanding how the brain works at the level of neural circuits. In this talk, the fundamental challenges of deep tissue, high-resolution optical imaging are discussed. New technologies for in vivo structural and functional imaging of mouse brain using long wavelength excitation and three-photon microscopy will be presented. We will illustrate the requirements for imaging the dynamic neuronal activity at the cellular level over a large area and depth in awake and behaving animals, and show applications where 3-photon microscopy outperforms conventional 2-photon microscopy in both signal strength and image contrast. Finally, we will discuss several future directions, including adaptive optics and new laser sources, to further improve the imaging depth and speed in biological tissues.